tak1 thr 184 187 Search Results


nbp1 28904g tak1 thr184 diffuse cytoplasmic rabbit polyclonal n a  (Novus Biologicals)
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Novus Biologicals nbp1 28904g tak1 thr184 diffuse cytoplasmic rabbit polyclonal n a
Nbp1 28904g Tak1 Thr184 Diffuse Cytoplasmic Rabbit Polyclonal N A, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal phospho-tak1 (thr 187  (Bioss)
90
Bioss rabbit monoclonal phospho-tak1 (thr 187
A: RPE cells were treated with TGF-β1 (2.5ng/ml) for the indicated times or left untreated. The cells were then immunostained with phospho-Thr 187 <t>TAK1</t> antibodies (green) and DAPI (blue) as described in Materials and Methods. Representative photographs of three independent experiments. Scale bar is 10μm for all images. B : The histogram demonstrating pixel intensity, measured using Image-J software, is based on three independent experiments. (Number of cells: Control 0 = 24, Control 4 hours = 28, control 24 hours = 21, control 48 hours = 25; TGF-β1 4 hours = 26, TGF-β1 24 hours = 21, TGF-β1 48 hours = 22). Statistics were computed using student t-test (Two tailed distribution equal variance). Data is expressed as the Mean±SD.
Rabbit Monoclonal Phospho Tak1 (Thr 187, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphor tak1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc phosphor tak1
Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and <t>TAK1,</t> or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.
Phosphor Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho tak1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc phospho tak1
Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and <t>TAK1,</t> or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.
Phospho Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho tak1/product/Cell Signaling Technology Inc
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rabbit anti phospho tak1 thr187 antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti phospho tak1 thr187 antibody
Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and <t>TAK1,</t> or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.
Rabbit Anti Phospho Tak1 Thr187 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tak1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc tak1
Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and <t>TAK1,</t> or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.
Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho tak1  (Proteintech)
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Proteintech phospho tak1
Fig. 5. Effects of phospholipase C (PLC) (A) and protein kinase C (PKC) (B) antagonists on Wnt agonist 1-induced <t>TAK1</t> banding 1 (TAB1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.
Phospho Tak1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho-tak1 (thr-187) ab antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc phospho-tak1 (thr-187) ab antibody
Fig. 5. Effects of phospholipase C (PLC) (A) and protein kinase C (PKC) (B) antagonists on Wnt agonist 1-induced <t>TAK1</t> banding 1 (TAB1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.
Phospho Tak1 (Thr 187) Ab Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho tak1 thr184 187 cell signaling technology  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti phospho tak1 thr184 187 cell signaling technology
Fig. 5. Effects of phospholipase C (PLC) (A) and protein kinase C (PKC) (B) antagonists on Wnt agonist 1-induced <t>TAK1</t> banding 1 (TAB1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.
Anti Phospho Tak1 Thr184 187 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p tak1 thr187  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology p tak1 thr187
Impact of Takinib administration on p <t>-TAK1</t> and TAK1 levels at 24h post-TBI. p -TAK1 and TAK1 were significantly upregulated 24 h following a TBI. Takinib treatment suppressed p -TAK1 and TAK1 levels post-24 h following a TBI, contrary to the control group. No significant variations were observed among the two TBI groups. Quantitative findings utilized the format of mean ± standard deviation (n = 6); ns, no significance, P > 0.05; *, P < 0.05 versus the control group; #, P < 0.05 versus the TBI + vehicle group. Original images of Western blots are presented in Supplementary fig. 1.
P Tak1 Thr187, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho tak1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti phospho tak1
Impact of Takinib administration on p <t>-TAK1</t> and TAK1 levels at 24h post-TBI. p -TAK1 and TAK1 were significantly upregulated 24 h following a TBI. Takinib treatment suppressed p -TAK1 and TAK1 levels post-24 h following a TBI, contrary to the control group. No significant variations were observed among the two TBI groups. Quantitative findings utilized the format of mean ± standard deviation (n = 6); ns, no significance, P > 0.05; *, P < 0.05 versus the control group; #, P < 0.05 versus the TBI + vehicle group. Original images of Western blots are presented in Supplementary fig. 1.
Anti Phospho Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho tak1 thr187  (Proteintech)
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Proteintech phospho tak1 thr187
Impact of Takinib administration on p <t>-TAK1</t> and TAK1 levels at 24h post-TBI. p -TAK1 and TAK1 were significantly upregulated 24 h following a TBI. Takinib treatment suppressed p -TAK1 and TAK1 levels post-24 h following a TBI, contrary to the control group. No significant variations were observed among the two TBI groups. Quantitative findings utilized the format of mean ± standard deviation (n = 6); ns, no significance, P > 0.05; *, P < 0.05 versus the control group; #, P < 0.05 versus the TBI + vehicle group. Original images of Western blots are presented in Supplementary fig. 1.
Phospho Tak1 Thr187, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A: RPE cells were treated with TGF-β1 (2.5ng/ml) for the indicated times or left untreated. The cells were then immunostained with phospho-Thr 187 TAK1 antibodies (green) and DAPI (blue) as described in Materials and Methods. Representative photographs of three independent experiments. Scale bar is 10μm for all images. B : The histogram demonstrating pixel intensity, measured using Image-J software, is based on three independent experiments. (Number of cells: Control 0 = 24, Control 4 hours = 28, control 24 hours = 21, control 48 hours = 25; TGF-β1 4 hours = 26, TGF-β1 24 hours = 21, TGF-β1 48 hours = 22). Statistics were computed using student t-test (Two tailed distribution equal variance). Data is expressed as the Mean±SD.

Journal: PLoS ONE

Article Title: TGF-β1 Induced Transdifferentiation of RPE Cells is Mediated by TAK1

doi: 10.1371/journal.pone.0122229

Figure Lengend Snippet: A: RPE cells were treated with TGF-β1 (2.5ng/ml) for the indicated times or left untreated. The cells were then immunostained with phospho-Thr 187 TAK1 antibodies (green) and DAPI (blue) as described in Materials and Methods. Representative photographs of three independent experiments. Scale bar is 10μm for all images. B : The histogram demonstrating pixel intensity, measured using Image-J software, is based on three independent experiments. (Number of cells: Control 0 = 24, Control 4 hours = 28, control 24 hours = 21, control 48 hours = 25; TGF-β1 4 hours = 26, TGF-β1 24 hours = 21, TGF-β1 48 hours = 22). Statistics were computed using student t-test (Two tailed distribution equal variance). Data is expressed as the Mean±SD.

Article Snippet: Slides were stained with rabbit monoclonal phospho-TAK1 (Thr 187) (Bioss, Woburn, MA, USA), α-SMA (Sigma Aldrich, Rehovot, Israel), Isotype IgG2a, primary antibodies and then with Alexa Fluor 488-conjugated secondary antibodies (Millipore, Billerica MA, USA).

Techniques: Software, Two Tailed Test

Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and TAK1, or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.

Journal: Journal of Functional Foods

Article Title: Anti-inflammatory effects and molecular mechanisms of loquat (Eriobotrya japonica) tea

doi: 10.1016/j.jff.2013.11.019

Figure Lengend Snippet: Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and TAK1, or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.

Article Snippet: Antibodies against phosphor-ERK1/2, phosphor-p38 kinase, phosphor-JNK, ERK1/2, p38 kinase, JNK, phosphor-TAK1 (Thr184/187), phospho-IKKa/b (ser176/180), IjB-a and phospho-IRF3 were purchased from Cell Signaling Technology, Beverly, MA, USA.

Techniques: Inhibition, Phospho-proteomics, Western Blot, Control

Fig. 5. Effects of phospholipase C (PLC) (A) and protein kinase C (PKC) (B) antagonists on Wnt agonist 1-induced TAK1 banding 1 (TAB1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 5. Effects of phospholipase C (PLC) (A) and protein kinase C (PKC) (B) antagonists on Wnt agonist 1-induced TAK1 banding 1 (TAB1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques: Expressing, Control

Fig. 6. Effects of phospholipase C (PLC) (A, B) and protein kinase C (PKC) (C, D) antagonists on Wnt agonist 1-induced TAK1 expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983; p-TAK1, phospho-TAK1; t- TAK1, total-TAK1. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 6. Effects of phospholipase C (PLC) (A, B) and protein kinase C (PKC) (C, D) antagonists on Wnt agonist 1-induced TAK1 expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983; p-TAK1, phospho-TAK1; t- TAK1, total-TAK1. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques: Expressing, Control

Fig. 7. Effects of phospholipase C (PLC) (A), protein kinase C (PKC) (B) and TAK1 (C) antagonists on Wnt agonist 1-induced ATF2 expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983, TI, TAK1 inhibitor, an inhibi- tor of TAK1; p-ATF2, phospho-ATF2; t-ATF2, total-ATF2. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 7. Effects of phospholipase C (PLC) (A), protein kinase C (PKC) (B) and TAK1 (C) antagonists on Wnt agonist 1-induced ATF2 expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983, TI, TAK1 inhibitor, an inhibi- tor of TAK1; p-ATF2, phospho-ATF2; t-ATF2, total-ATF2. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques: Expressing, Control

Fig. 8. Effects of phospholipase C (PLC), protein kinase C (PKC) and TAK1 antagonists on Wnt agonist 1-induced T cell factor (TCF)3 (A–C) and TCF4 (D– F) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983, TI, TAK1 inhibitor. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 8. Effects of phospholipase C (PLC), protein kinase C (PKC) and TAK1 antagonists on Wnt agonist 1-induced T cell factor (TCF)3 (A–C) and TCF4 (D– F) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983, TI, TAK1 inhibitor. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques: Expressing, Control

Fig. 9. Effects of phospholipase C (PLC) (A), protein kinase C (PKC) (B) and TAK1 (C) antagonists on Wnt agonist 1-induced lymphoid enhancer factor 1 (LEF1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983, TI, TAK1 inhibitor. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 9. Effects of phospholipase C (PLC) (A), protein kinase C (PKC) (B) and TAK1 (C) antagonists on Wnt agonist 1-induced lymphoid enhancer factor 1 (LEF1) expression in beating rat atria. Data were expressed as means ± SE. n = 5. Cont, control; Wnta1, Wnt agonist 1; U, U73122; Go, Gö6983, TI, TAK1 inhibitor. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques: Expressing, Control

Fig. 10. Effects of TAK1 antagonist on Wnt agonist 1-induced atrial natriuretic peptide (ANP) secretion (A) and dynamics (B) in beat- ing rat atria. Data were expressed as means ± SE. n = 6. Cont, control; Wnta1, Wnt agonist 1; TI, TAK1 inhibitor. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 10. Effects of TAK1 antagonist on Wnt agonist 1-induced atrial natriuretic peptide (ANP) secretion (A) and dynamics (B) in beat- ing rat atria. Data were expressed as means ± SE. n = 6. Cont, control; Wnta1, Wnt agonist 1; TI, TAK1 inhibitor. *p < 0.05 vs. control, #p < 0.05 vs. Wnta1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques: Control

Fig. 11. Schematic mechanisms by which Wnt agonist 1 (Wnta1) regulates atrial atrial natriuretic peptide (ANP) secretion and me- chanical dynamics. PLC, phospholipase C; PKC, protein kinase C; TAB1, TAK1 banding 1; TCF, T cell factor; LEF1, lymphoid enhancer factor 1.

Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: The WNT/Ca 2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria.

doi: 10.4196/kjpp.2022.26.6.469

Figure Lengend Snippet: Fig. 11. Schematic mechanisms by which Wnt agonist 1 (Wnta1) regulates atrial atrial natriuretic peptide (ANP) secretion and me- chanical dynamics. PLC, phospholipase C; PKC, protein kinase C; TAB1, TAK1 banding 1; TCF, T cell factor; LEF1, lymphoid enhancer factor 1.

Article Snippet: The antibodies used in this study were as follows: anti-β-actin (1:1,000, AP0060; Bioworld Technology, Wuhan, China), polyclonal anti-PKCβ (1:1,000, ENT3756; Elabscience, Wuhan, China), polyclonal anti-PKCγ (1:1,000, E-AB-13012; Elabscience), polyclonal TAB1 antibody WNT/Ca2+ signaling in isolated rat atria Korean J Physiol Pharmacol 2022;26(6):469-478www.kjpp.net 471 (1:1,000, DF7471; Affinity, Changzhou, China), phospho-TAK1 (Thr184/Thr187) antibody (1:1,000, AF4379), polyclonal antiTAK1 (1:500, 12330-2-AP; Proteintech, Wuhan, China), phosphoATF2 (T69/T71) antibody (1:1,000, AP0525; ABclonal, Wuhan, China), polyclonal anti-ATF2 (1:1,000, 11908-1-AP; Proteintech), anti-LEF1 Antibody (1:1,000, DF7570; Affinity), polyclonal antiTCF3 (1:1,000, 14519-1-AP; Proteintech), polyclonal anti-TCF4 (1:2,000, 13838-1-AP; Proteintech), and horseradish peroxidaseconjugated anti-rabbit secondary antibody (1:3,000, AP132P, Nachuan Biotech, Changchun, China).

Techniques:

Impact of Takinib administration on p -TAK1 and TAK1 levels at 24h post-TBI. p -TAK1 and TAK1 were significantly upregulated 24 h following a TBI. Takinib treatment suppressed p -TAK1 and TAK1 levels post-24 h following a TBI, contrary to the control group. No significant variations were observed among the two TBI groups. Quantitative findings utilized the format of mean ± standard deviation (n = 6); ns, no significance, P > 0.05; *, P < 0.05 versus the control group; #, P < 0.05 versus the TBI + vehicle group. Original images of Western blots are presented in Supplementary fig. 1.

Journal: Heliyon

Article Title: Neuroprotective effects of takinib on an experimental traumatic brain injury rat model via inhibition of transforming growth factor beta-activated kinase 1

doi: 10.1016/j.heliyon.2024.e29484

Figure Lengend Snippet: Impact of Takinib administration on p -TAK1 and TAK1 levels at 24h post-TBI. p -TAK1 and TAK1 were significantly upregulated 24 h following a TBI. Takinib treatment suppressed p -TAK1 and TAK1 levels post-24 h following a TBI, contrary to the control group. No significant variations were observed among the two TBI groups. Quantitative findings utilized the format of mean ± standard deviation (n = 6); ns, no significance, P > 0.05; *, P < 0.05 versus the control group; #, P < 0.05 versus the TBI + vehicle group. Original images of Western blots are presented in Supplementary fig. 1.

Article Snippet: The blot was then subjected to overnight incubation at 4 °C with primary TAK1 and p -TAK1 (Thr187) antibodies (1:200; Santa Cruz Biotech., CA), ZO-1, an inhibitor of NF-κB (IκB-α), cleaved caspase-3 (1:500; Cell Signaling Technology, MA), claudin-5, p65 (1:1,000, Abcam), β-actin, tubulin and histone 3 (1:1000; Proteintech, USA), Bcl-2 and Bax (1:500; Santa Cruz Biotech., CA).

Techniques: Control, Standard Deviation, Western Blot